pressure during the experiment, and for collecting gas samples at
the start of the experiment and every 24 h for 10 times. CH4 was
measured by a portable gas multiplex detector (VISA, GMI, UK). The
total amount of CH4 found in the treatment with PNSB inoculum
was compared with the total amount of CH4 in the control that had
no addition of PNSB. The effect of PNSB in reducing CH4 production