FiveforwardandsixreversenosZprimercandidates
were found (Table 3, Fig. 1c). For the nosZ primers,
Nos661FandNos1773R,smallmodificationsweretes-
tedbutinTable1onlythebestresult,whichwasfrom
theoriginalprimers,isshown.NoneofthenosZprimer
sets resulted in fragments from the non-denitrifying
strains.Thebestcombinationwasshowntobeprimers
nosZ-F[18]andnosZ1622R,whichwasdesignedinthe
present study. Together they amplified the correct
fragment in 25 of the 28 denitrifying bacterial strains
andinallenvironmentalsamples.