Primer set used for phenylalanine ammonia-lyase (PAL), cinnamate
4-hydroxylase (C4H), 4-coumarate:CoA ligase (4CL), chalcone
synthase (CHS), flavanone 3-hydroxylase (F3H), dihydroflavonol 4-
reductase (DFR), leucoanthocyanidin dioxygenase (LDOX, also
called anthocyanidin synthase, ANS; Appelhagen et al., 2011) gene
expression analysis were designed on the sequences reported by
Dardick et al. (2010). Chalcone isomerase (CHI) gene specific primers
were designed on the sequences reported by Zhou et al. (2013).
Average expression for each RNA was determined from the highly
consistent triplicate reactions, with the range of the reactions
never more than 0.5 threshold cycle (Ct, a measure of the time at
which the PCR product reaches a fixed threshold). Molecular analysis
were on samples collected at t0 and after 36 h for both UV-B
treated and control fruits.
Primer set used for phenylalanine ammonia-lyase (PAL), cinnamate4-hydroxylase (C4H), 4-coumarate:CoA ligase (4CL), chalconesynthase (CHS), flavanone 3-hydroxylase (F3H), dihydroflavonol 4-reductase (DFR), leucoanthocyanidin dioxygenase (LDOX, alsocalled anthocyanidin synthase, ANS; Appelhagen et al., 2011) geneexpression analysis were designed on the sequences reported byDardick et al. (2010). Chalcone isomerase (CHI) gene specific primerswere designed on the sequences reported by Zhou et al. (2013).Average expression for each RNA was determined from the highlyconsistent triplicate reactions, with the range of the reactionsnever more than 0.5 threshold cycle (Ct, a measure of the time atwhich the PCR product reaches a fixed threshold). Molecular analysiswere on samples collected at t0 and after 36 h for both UV-Btreated and control fruits.
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