The effects of pure oxygen on pericarp browning, reactive oxygen species (ROS) metabolism, antioxidant
enzyme and antioxidant activity of harvested litchi fruit were investigated. Applica tion of pure oxyge n
signi fi cantly prevented pericarp browning and delayed the increase in membrane permeability of litchi fruit
during storage. Litchi fruit exposed to pure oxyge n showed a lower level of lip id peroxides, compared to
control fruit, with the delay in the increases of both H 2O 2 content and superoxide produ ction rate.
Furthermore, it was found that the treatment with pure oxygen induced the activities of superoxide
dismutase (SOD), ascorbated peroxidase (APX) and catalase (CAT), which could be bene fi cial in scavenging of
H 2O 2 and superoxide and alleviatin g lipid peroxidation. In addition, antioxidant ability (reducing power and
free-radical scavenging activity against DPPH radical, superoxide anions and hydroxyl radical) of methanol
extracts from litchi fruit pericarp declined gradually, with decreasing contents of anthocyanin s and phenolic
compounds, as storage time of the fruit progressed. There was a linear relationship between the contents of
either anthocyanin s or phenolic compounds and antioxidant abi lity or free radical scavenging activity.
Treatment with pure oxygen markedly increased antioxi dant ability, which was related to higher levels of
anthocyanin s and phenol ic compou nds, compared with those of control fruit. It is suggested that enhanced
antioxidant activity and antioxidant enzyme induc ed by pure oxygen may contribute to alleviating lipid
peroxidation and maintenance of membrane integrity, which reduced decompartm entation of enzymes and
substrates, resulting in enzymatic browning.
The effects of pure oxygen on pericarp browning, reactive oxygen species (ROS) metabolism, antioxidant
enzyme and antioxidant activity of harvested litchi fruit were investigated. Applica tion of pure oxyge n
signi fi cantly prevented pericarp browning and delayed the increase in membrane permeability of litchi fruit
during storage. Litchi fruit exposed to pure oxyge n showed a lower level of lip id peroxides, compared to
control fruit, with the delay in the increases of both H 2O 2 content and superoxide produ ction rate.
Furthermore, it was found that the treatment with pure oxygen induced the activities of superoxide
dismutase (SOD), ascorbated peroxidase (APX) and catalase (CAT), which could be bene fi cial in scavenging of
H 2O 2 and superoxide and alleviatin g lipid peroxidation. In addition, antioxidant ability (reducing power and
free-radical scavenging activity against DPPH radical, superoxide anions and hydroxyl radical) of methanol
extracts from litchi fruit pericarp declined gradually, with decreasing contents of anthocyanin s and phenolic
compounds, as storage time of the fruit progressed. There was a linear relationship between the contents of
either anthocyanin s or phenolic compounds and antioxidant abi lity or free radical scavenging activity.
Treatment with pure oxygen markedly increased antioxi dant ability, which was related to higher levels of
anthocyanin s and phenol ic compou nds, compared with those of control fruit. It is suggested that enhanced
antioxidant activity and antioxidant enzyme induc ed by pure oxygen may contribute to alleviating lipid
peroxidation and maintenance of membrane integrity, which reduced decompartm entation of enzymes and
substrates, resulting in enzymatic browning.
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The effects of pure oxygen on pericarp browning, reactive oxygen species (ROS) metabolism, antioxidant
enzyme and antioxidant activity of harvested litchi fruit were investigated. Applica tion of pure oxyge n
signi fi cantly prevented pericarp browning and delayed the increase in membrane permeability of litchi fruit
during storage. Litchi fruit exposed to pure oxyge n showed a lower level of lip id peroxides, compared to
control fruit, with the delay in the increases of both H 2O 2 content and superoxide produ ction rate.
Furthermore, it was found that the treatment with pure oxygen induced the activities of superoxide
dismutase (SOD), ascorbated peroxidase (APX) and catalase (CAT), which could be bene fi cial in scavenging of
H 2O 2 and superoxide and alleviatin g lipid peroxidation. In addition, antioxidant ability (reducing power and
free-radical scavenging activity against DPPH radical, superoxide anions and hydroxyl radical) of methanol
extracts from litchi fruit pericarp declined gradually, with decreasing contents of anthocyanin s and phenolic
compounds, as storage time of the fruit progressed. There was a linear relationship between the contents of
either anthocyanin s or phenolic compounds and antioxidant abi lity or free radical scavenging activity.
Treatment with pure oxygen markedly increased antioxi dant ability, which was related to higher levels of
anthocyanin s and phenol ic compou nds, compared with those of control fruit. It is suggested that enhanced
antioxidant activity and antioxidant enzyme induc ed by pure oxygen may contribute to alleviating lipid
peroxidation and maintenance of membrane integrity, which reduced decompartm entation of enzymes and
substrates, resulting in enzymatic browning.
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