0.5–10 lM, wherein the linear regression equation was I0–I = 13.72
[PPi] (lM) + 8.743 with a correlation coefficient (r
2
) of 0.9940. The
limit of detection (LOD) and limit of quantitation (LOQ) were then
investigated by determining the base fluorescence intensity (I0) of
the sensor probe ([Cys-CdS QDs]-Fe3+) 10 times. The LOD was calculated
as the concentration of PPi producing the fluorescence
intensity equal to I0 3 the standard deviation of I0, whereas
the LOQ was also calculated as the concentration of PPi affording
a fluorescence intensity equal to I0 10 the standard deviation
of I0 [45]. The LOD and LOQ of the proposed sensor under the optimized
conditions were found to be 0.11 lM and 2.78 lM, respectively.
These results indicate that the developed sensor was
highly sensitive and that it has the potential to detect phosphate-containing
compounds at very low concentration levels. Furthermore,
the repeatability of the proposed sensor was evaluated.
The relative standard deviation from the detection of 5 lM of PPi
using five replicates was found to be 2.44%. This result suggested
that the developed sensor showed very good repeatability.
Application of the proposed sensor for the detection of PPi in urine
samples
To demonstrate the feasibility of the proposed sensor in real
applications, urine was used as a representative clinical sample.
A urine sample from a healthy volunteer was collected and filtered
through a Whatman No. 42 filter. Then, the filtered urine sample
was diluted 100 times with 0.1 M Tris–HCl buffer pH 7.5 and
spiked with PPi at three different concentration levels (2, 5, and
8 lM). The sensor probe was added to the sample and incubated
0.5–10 lM, wherein the linear regression equation was I0–I = 13.72
[PPi] (lM) + 8.743 with a correlation coefficient (r
2
) of 0.9940. The
limit of detection (LOD) and limit of quantitation (LOQ) were then
investigated by determining the base fluorescence intensity (I0) of
the sensor probe ([Cys-CdS QDs]-Fe3+) 10 times. The LOD was calculated
as the concentration of PPi producing the fluorescence
intensity equal to I0 3 the standard deviation of I0, whereas
the LOQ was also calculated as the concentration of PPi affording
a fluorescence intensity equal to I0 10 the standard deviation
of I0 [45]. The LOD and LOQ of the proposed sensor under the optimized
conditions were found to be 0.11 lM and 2.78 lM, respectively.
These results indicate that the developed sensor was
highly sensitive and that it has the potential to detect phosphate-containing
compounds at very low concentration levels. Furthermore,
the repeatability of the proposed sensor was evaluated.
The relative standard deviation from the detection of 5 lM of PPi
using five replicates was found to be 2.44%. This result suggested
that the developed sensor showed very good repeatability.
Application of the proposed sensor for the detection of PPi in urine
samples
To demonstrate the feasibility of the proposed sensor in real
applications, urine was used as a representative clinical sample.
A urine sample from a healthy volunteer was collected and filtered
through a Whatman No. 42 filter. Then, the filtered urine sample
was diluted 100 times with 0.1 M Tris–HCl buffer pH 7.5 and
spiked with PPi at three different concentration levels (2, 5, and
8 lM). The sensor probe was added to the sample and incubated
การแปล กรุณารอสักครู่..

0.5–10 lM, wherein the linear regression equation was I0–I = 13.72
[PPi] (lM) + 8.743 with a correlation coefficient (r
2
) of 0.9940. The
limit of detection (LOD) and limit of quantitation (LOQ) were then
investigated by determining the base fluorescence intensity (I0) of
the sensor probe ([Cys-CdS QDs]-Fe3+) 10 times. The LOD was calculated
as the concentration of PPi producing the fluorescence
intensity equal to I0 3 the standard deviation of I0, whereas
the LOQ was also calculated as the concentration of PPi affording
a fluorescence intensity equal to I0 10 the standard deviation
of I0 [45]. The LOD and LOQ of the proposed sensor under the optimized
conditions were found to be 0.11 lM and 2.78 lM, respectively.
These results indicate that the developed sensor was
highly sensitive and that it has the potential to detect phosphate-containing
compounds at very low concentration levels. Furthermore,
the repeatability of the proposed sensor was evaluated.
The relative standard deviation from the detection of 5 lM of PPi
using five replicates was found to be 2.44%. This result suggested
that the developed sensor showed very good repeatability.
Application of the proposed sensor for the detection of PPi in urine
samples
To demonstrate the feasibility of the proposed sensor in real
applications, urine was used as a representative clinical sample.
A urine sample from a healthy volunteer was collected and filtered
through a Whatman No. 42 filter. Then, the filtered urine sample
was diluted 100 times with 0.1 M Tris–HCl buffer pH 7.5 and
spiked with PPi at three different concentration levels (2, 5, and
8 lM). The sensor probe was added to the sample and incubated
การแปล กรุณารอสักครู่..
