The antibacterial activity of the essential oils was
tested by disc-diffusion and broth microdilution methods.
In the disc-diffusion method, a sterile Whatman
disc (6 mm) saturated with 10 µl of essential oil 17 was
put on a lawn of a bacterial inoculum which has a
turbidity in 1% (w/v) tryptone water equated to a McFarland
No 0.5 standard (approximately 108 CFU/ml).
All values of inhibition zones were expressed as
mean ± standard deviation. S. pneumoniae ATCC
49619, H. influenzae ATCC 49247, and S. aureus
ATCC 25923 were used as quality controls. For broth