The soybean CHS7/8 (v1.1 Glyma01g43880 and Glyma11g01350) and rice CHS (MSU release 7.0 Os11g0530600) sequences were aligned using a global alignment in Geneious (Kearse et al. 2012) with the following parameters: cost matrix 65 %, gap open penalty 12, and gap extension penalty 3.