•3.1.2 Analytical Column
•The most commonly used analytical columns are 10, 15, or 25 cm long with an internal diameter 4.6 or 5 mm.
•Analytical columns are generally packed with 3,5, or 10 μmparticles and are operated at flow rates of 1-2 ml/min. Short (3 cm) columns, packed with ≤ 3 μmparticles, are used for fast separations; for example, in method development or process monitoring.