In the present study, E. bieneusi was found in 54 of the 91 pigs examined. This report constitutes the first report of E. bieneusi in pigs in Brazil. There is only another prevalence study conducted in pigs in the American continent for E. bienesi in pigs. In that study, feces and gall bladders obtained at a slaughterhouse from 202 healthy pigs in the United States were examined with 32% of the pigs positive for E. bieneusi in feces and/or bile specimens