Antagonistic effect of pseudomonas isolates
against Fusarium oxysporum was tested by
dual culture method outlined by Skidmore and
Dickinson (1976) For this, three predominant
isolates of pseudomonas selected from each
location were used along with commercial
culture of pseudomonas. Mycelial discs (6
mm) of pathogen from seven day old culture
grown on PDA was inoculated aseptically on
one scale of petridishes containing PDA and
incubated at 28 ± 2oC for 24 hours. After this
Pseudomonas was inoculated in the same
petridishes 3.5 cm away from the pathogen
and incubated for 5 days. Three replications
were maintained for each isolate. Pathogen
grown in monoculture served as control.
Growth measurements were taken at regular
intervals after 24 h of inoculation of
antagonistic for four days. Nature of reaction
of the antagonist on the pathogen were
recorded.