Aires province, were housed in a 1 m2 terrarium containing substrate and water simulating their natural habitat at the Facultad de Ciencias Exactas of the Universidad Nacional La Plata (UNLP) for a 5 year period. They were kept at 25°C ± 1 with a photoperiod of 16L:8D. On August 26, 2009 they were injected with AMPHIPLEX at 10:00 in the morning.