and incubated at
30 C without agitation. Aliquots were taken from every flask and
used to measure the absorbance at 600 nm hourly up to 12 h. Once
the tested microorganisms had reached the exponential growth
phase (appr. 3 h), partially purified bacteriocin ST71KS was added
to one of each pair of flasks (20%, v/v). The flasks with no added
bacteriocin were used as controls.
2.5.3. Cell lysis, measured by extracellular levels of b-galactosidase