The isolated
bacteria was inoculums in CMC broth, fungi isolated were inoculums in PDB+0.5% CMC, then
incubated at 37 °C for 72 h with shaking speed 140 rpm. Culture broth was centrifugation at 6,000
rpm at room temperature for 10 min to obtain culture supernatants which were later measured
cellulase activity by DNS method. The optical density was read at 520 nm by a spectrophotometer.