2.1. Microorganisms
A. awamori and A. oryzae were purchased from Shanghai Beinuo
Biotechnology Co., Ltd. and used for production of glucoamylase
and protease, respectively. They were prepared according to the
previous publication (Pleissner et al., 2014) and stored at 80 C
until used for solid-state fermentation.
The anaerobic sludge was collected from a local municipal
wastewater treatment plant and screened by a sieve (diameter:
2 mm) to eliminate large particulate materials. It was heat pretreated
in a water bath at temperature of 100 C for 6 h to inhibit
the methane-producing bacteria activity (Ren et al., 2012). The
heat pretreated sludge was then used as inoculum for fermentative
hydrogen production.
2