Five fish were exsanguinated
by CVP and five by CT. For each fish, the first
I .5 ml blood sampled was called “aliquot l”,
the following 1.5 ml blood was called “aliquot
2”, a.s.o., until no more blood was available.
Blood was mixed with Na,-EDTA, 1.3 mg/ml
(20 p I/sample). The total sampling time for each
fish was approximately 6 min.