The reaction mixture was generated by adding 30 lL of 2-deoxy-2-ribose (17 mM), 30 lL of the extract, 30 lL of 1.2 mM EDTA, 60 lL of 0.3 mM FeCl3, 30 lL of 34 mM hydrogen peroxide (H2O2), and 60 lL of 0.6 mM ascorbic acid. The reaction was performed at 37 C for 1 h. Thereafter, 150 lL of 1% (w/v) thiobarbituric acid (TBA) and 300 lL of 2.8% (w/v) trichloroacetic acid (TCA) were added to the mixture, which was then incubated at 100 C for 15 min. A