A blank composed of nuclease-free water and master mix was included as a control. Samples were processed using the default run module Fragment Analysis36_POP7_1 and dye set G5 (POP-7 Polymer, 23 second injection, 1.2 kV injection voltage, 60 8C, run time 20 min,filter set G5). Results were analyzed using GeneMapper Software Version 4.0 (Applied Biosystems), AFLP default setting.