DNA sequences of PCR amplicons produced by 20 previously reported STS primer pairs (12, 13, 17) were compared against all sequences in the nonredundant databases using the BLASTX and BLASTN programs.
The amplicon sizes ranged from about 450 to 1800 bp in length.
However, no candidate gene of any known function possibly related to quality traits was found among the sequences derived from STS primers.
Therefore, a total of 21 markers,including SSIIa (3), were used directly in this study (Table 1)as 5 of the markers listed inTable 1from Bao et al.(2) were not polymorphic among the 22 varieties.