The presence of auxin in defined combinations with
cytokinins in the culture medium is also necessary to
obtain adventitious shoot formation (Caboni and Tonelli,
1999). Presence of BAP in the culture medium was
necessary for the shoot regeneration, although concentrations
higher than 4.44 uM reduced the shoot regeneration
frequency (Hodson et al., 2008). Waseem et al.,
(2006) inoculated chrysanthemum nodal segments in
Murashige and Skoog (MS) media supplemented with
different concentrations of indole acetic acid (IAA),
naphthalene acetic acid (NAA) and indole butyric acid
(IBA) and reported that 0.3 mg/l IAA, 0.5 mg/l NAA and
0.3 mg/l IBA showed their superiority over all their other
respective concentrations, when used alone. Therefore,
the attempts were made to determine the effect of
different growth regulators on the shoot proliferation and
rooting of chrysanthemum plantlets using nodal segments
explant.