The objective of this studywas to evaluate the potential of fluorescence spectroscopy to identify differentmuscles
and to predict some physicochemical and rheological parameters. Samples were taken from three muscles
(Semitendinosus, Rectus abdominis and Infraspinatus) of Charolais breed. Dry matter content, fat content, protein
content, texture and collagen content were determined. Moreover emission spectra were recorded in the range
of 305–400 nm, 340–540 nm and 410–700 nm by fixing the excitation wavelength at 290, 322 and 382 nm,
respectively. The results obtained were evaluated by partial least square discriminant analysis and partial least
square regression. Results of our research work show that front-face fluorescence spectroscopy and
chemometrics offer significant potential for the development of rapid and non-destructive methods for the
identification and characterization of muscles.