Three grams of uncooked shrimp meat were homogenized with 30 ml of 10 g/100 ml SDS solution at room temperature
using a homogenizer (IKA Labortechnik, Selangor, Malaysia). The homogenate were incubated at 85 C for 1 h to dissolve the total proteins, and centrifuged. The supernatants were collected and the protein concentrations were determined using Biuret method