POD activity was
determined according to the method of Yang et al. (2009) with
some modifications. The reaction mixture consisted of 0.3 mL of
6 mmol L1 guaiacol, 1.7 mL of 5 mmol L1 H2O2, and 1 mL of crude
enzyme extract. Increases in absorbance at 470 nm at intervals of
30 s were recorded spectrophotometrically