SlCRF3 and SlCRF5 expression analysis in various organs by RT-PCR was started with a one-step cycle of 2 min at 95◦C, followed by 29 cycles of 30 s at 94◦C, 45 s at 56◦C, and 50 s at 72◦C, and a 5 min final extension at 72◦C, using gene specific primer as mentioned in Shiet al. (2012)