In order to confirm that the decolourization of the ITE by C. globosum IMA1 was occurred by the biodegradation way or no,
the amount of the adsorbed dye on the mycelium at the end of incubations was measured using the spectrophotometric method. The results of this study showed that the percentages of dye adsorbed at the end of the biological treatment were 22% and 8% for static and stirred conditions, respectively. As to biodegradation, the COD removal yield could reach a high value of about 88.4%. In fact, stirring also enhanced nutritional requirements of the organisms that were used for its metabolism and then improved the biodegradation efficiency.