Early blight of tomato, which is mainly caused by Alternaria solani, is an important, globally distributeddisease.
We previously isolated an endophytic bacterium (BT4) from healthy tomatoes that has high antifungalactivity against A. solani. Industrial applications of antagonistic bacteria require the optimization of culture conditions for growth and antifungal activity.
Thus, in this study, the optimum medium composition
and culture conditions for BT4 were determined using the ‘‘one factor at a time’’ method and an orthogonal design.