Previous works have shown that high Se concentrations were
toxic to mushroom formation, as observed by Gaso et al. (2000)
and Hartikainen (2005). Se concentration higher than 25.4 mg kg1
was a good stimulus for the 3rd flush but not for the others, causing a reduction in the BE on the first and second flushes (Fig. 2).
These results may be due to a reduction of Se concentration in
the substrate, throughout flushes, leading to an alleviation of toxicity and enhanced mushroom formation.