As concerned to the culture linear response to blue and green LEDs, the highest bud numbers (11.7 and 11.8, respectively) were found with 45g L-1 of sucrose. Therefore, in this experiment, the optimized bud number varied from 8.5 to 14.7 depending on the light source, evidencing the adequate adjustment between light source and sucrose concentration for sugarcane micropropagation, since values between 6 and 10 buds have been reported in the literature (JALAJA et al., 2008)