All the amplification products were electrophoresed in a 1%
agarose gel in TAE buffer and stained with ethidium bromide. Gels
were visualized under UV light in a G-Box Syngene-Genesis 10 UV
Scanner (Cambridge, United Kingdom). Fragment sizes were estimated
by comparison against a DNA ladder (NZYDNA Ladder 5,
NZYTech).
2.4. Yeast isolation