In order to investigate the GSH biosynthetic behavior
of S. cerevisiae WSH-J701, a time/course of cell growth
and total GSH concentration was monitored in a batch
culture (Fig. 1). GSH production was associated with
cell growth, the maximum total GSH concentration,
64.7 mg/l, was achieved after 32 h cultivation. Afterwards,
both the biomass concentration and total GSH
concentration decreased.