Effect on the Expression Levels of ERs, eNOS, and
TNF-α. To further investigate the involvement of the estrogenic
activity of C. comosa on vascular dysfunction induced by OVX,
we determined the expression level of ERs in blood vessels. As
seen in Figure 4A, the expression of ERα in OVX groups was
significantly reduced compared to the sham control. Treatment
with C. comosa powder and compound D3 at 100 mg/kg BW/
day significantly increased ERα expression (P < 0.05). In
contrast, the ERβ expression level was not significantly altered
by these treatments (Figure 4B).