This extends the contact time between the solvent and the sample
and allows it time to dissolve all of the fat contained in the sample. In order for the solvent to
thoroughly penetrate the sample it is necessary for the sample to be as finely comminuted as
possible. Both the solvent and the sample must be free of moisture to avoid coextraction of
water-soluble components in the sample such as carbohydrates, urea, lactic acid, glycerol, etc.
Low temperature is used to evaporate the ether and remove residual moisture to prevent
oxidation of the fat. Fat includes fatty acids, triglycerides, esters, long chain alcohols,
hydrocarbons, other glycol esters and sterols determined by the method. It is expressed as g fat
per 100g sample.