Conclusion
In this experiment, we tried to determine the best combination of NAA and BAP, under D+L condition for direct bud induction. Pre-culture of explants left in liquid medium for 1-2 days prior to transforming to regeneration medium has even improved the percentage of RE. Finally, the GA3 treatment produced effectively large shoots for micro-grafting. We also achieved a high RE (more than 80%), which is a prerequisite for any C. aurantium transformation protocol