For that, film rectangles (110 mm × 60 mm) were sealed in the side edges to form bags, and before the top was sealed(Fastvac, F 200 Flash, Brazil), 8 mL of sunflower oil was added to the bags. Two controls were used, which consisted of 20 mL of sunflower oil stored in closed plastic bottles (PLA) and in open glass Petri dishes (GLA).