Qualitative analysis - Thin layer Chromatography
The presence of capsaicinoid in the solvents was indentified using thin layer chromatography. It was performed on TLC silica gel 60 F254 aluminum sheets(Merck). The standard capsaicin at concentration of 1mg/ml was spotted as a reference on the TLC plate. Aliquots of 10µl of the two extracts were spotted onto the plates. The plates were dried in a hood for 10 min. Petroleum ether: Chloroform: Acetonitrile in the ratio of 40: 45: 15 as used as the mobile phase. The chromatogram was developed in iodine chamber and viewed under the UV light at 302 nm. The standard capsaicin Rf value was calculated and compared with the extracts.