Dry and complete shape brown rice of flo7 and wild
type were randomly selected, then ruptured artificially,
and the ruptured half brown rice of flo7 and wild type
were used. The half brown rice was affixed on sample
stage, the cross-section of which was upward, then the
cross-section of the half brown rice was gold-plated
by using vacuum coating machine (Hitachi, Japan).
Finally, 20 kV acceleration voltage was added in SEM,
and the observation and taking photos over different
position of the cross-section of the half brown rice
under SEM were conducted (Kang and Cheng, 2007).