fungal gene abundance were observed under biochar application
(Lu et al., 2015). Despite the increase of PLFAs, total CO2 emission decreased
for neural and alkaline soils in 500BC and 700BC, suggesting
other mechanisms such as the distribution of microbial communities
(G−/G+ ratio and F/B ratio) would also affect the mineralization of soil
carbon. As indicated in Fig. S4, the PLFA change was mainly related to
soil types rather than the kinds and application of biochar, and the effects
of soil types became more drastic as time goes on. The results
also suggest that the site effects are greater than biochar treatment, emphasizing
the importance of soil properties in the carbon sequestration
by biochar.