3. Results and discussion
3.1. Samples
Sampling scheme greatly affects the NIRs model generaliza- tion as the NIRs would be influenced by different batches of fermentation with different conditions, fermentors or different fermentation phases(Cervera et al., 2009). 15 batches of L. lae- tis subsp. fermentation were carried out under different condi- tions which were designed by Box Behnken design in 3 different fermentors. Broth samples were drawn out at aninterval of 1 h and the glucose concentration and their pH val ues were determined by reference methods immediately. The glucose and pH profiles of each batch of L. lactis subsp. fermentation are shown in Fig. I. As can be seen, glucose concentrations and pH values continually decreased since 2 h, and then, became stable after 7 h, which was indicating that 0-2 h was in incubative phase, 2-7 h was in growth phase and 7-10 h was in stable phase of L. lactis subsp. fermentation The calibration samples were collected from each phase of different batches of L. lactis subsp. fermentation, which was good for the generalization of the calibration models
3. Results and discussion 3.1. Samples Sampling scheme greatly affects the NIRs model generaliza- tion as the NIRs would be influenced by different batches of fermentation with different conditions, fermentors or different fermentation phases(Cervera et al., 2009). 15 batches of L. lae- tis subsp. fermentation were carried out under different condi- tions which were designed by Box Behnken design in 3 different fermentors. Broth samples were drawn out at aninterval of 1 h and the glucose concentration and their pH val ues were determined by reference methods immediately. The glucose and pH profiles of each batch of L. lactis subsp. fermentation are shown in Fig. I. As can be seen, glucose concentrations and pH values continually decreased since 2 h, and then, became stable after 7 h, which was indicating that 0-2 h was in incubative phase, 2-7 h was in growth phase and 7-10 h was in stable phase of L. lactis subsp. fermentation The calibration samples were collected from each phase of different batches of L. lactis subsp. fermentation, which was good for the generalization of the calibration models
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