Figure 2. Inhibition of TRPM8 activity by scPPX1 in intracellular Ca2+ measurements. Upper panels: Fluorescence measurements of intracellular Ca2+ concentration were performed on HEK-293 TRPM8 stable cell lines with transiently transfected GFP (0.2 μg) alone (panel A) or together with the scPPX1 clone (0.4 μg) (panel B). The summaries of averaged menthol responses are represented in panel C. Lower panels: Fluorescence measurements of intracellular Ca2+ signals were performed on F-11 neuronal cells with transiently transfected TRPM8 (0.4 μg) and GFP (0.2 μg) (panel D) or together with the scPPX1 clone (0.4 μg) (panel E). Thesummaries of averaged menthol responses are represented in panel F.