The present study describes a new approach to detect LMoV,based on a technique that has been used to detect many other anti-gens and virus-specific antibodies. The ICS test could detect the virus at 8.0 × 10−9 mg/mL, which was as sensitive as the quadruplex RT-PCR assay; compared with the latter, which was used as a reference test, the ICS test had a very high specificity (98.7%) and sensitivity (100%). There was significant agreement between the results obtained by quadruplex RT-PCR and the ICS tests ( = 0.982)with the field samples.