PLB induction under controlled conditions has been used to study the influence of abiotic and biotic factors ( Teixeira da Silva et al., 2006), medium constituents, carbon source, PGR, etc. ( Kishor and Devi, 2009 and Nasiruddin et al., 2003). In the present study, TDZ incorporated in MS medium was used for PLB induction and subsequent organogenesis in the segments of pseudostem explants ( Table 1). Depending upon the concentrations of TDZ in the medium, the pseudostem explants produced compact masses of green PLBs. Initially, small projections resulted from the explants within 4 weeks of culture which proliferated eventually into multiple shoots and plantlets within 8 weeks of the culture ( Fig. 1D–G). The highest explant response was 94.1% at 1.5 mg/l of TDZ. The synergistic effect of TDZ, in the present study, has been observed for efficient PLB induction from D. nobile explants so as to detect the immediate and long term effects of TDZ on the clonally propagated plants of this orchid species.