A one pot extraction from TSH was carried out and
compared with the three times repeated extraction. TS was
heated to separate TSH easily, then the partly dried TSH
was obtained and used for the extraction. The content of
polymeric PA in crude TSE was analyzed by HPLC and
determined using acTSE as a standard. The results are
summarized in Table 2. The content of polymeric PA in
fresh TSH reached about 39% (w/w), whereas in crude
TSE prepared by one pot extraction with ethanol/water
(3:2, v/v) at 60оC for 24 hours it was very high (about
94%, w/w). The recovery of polymeric PA from TSH with