sucrose, wheat flour and flour treatment agent E300 (ascorbic acid)} and shortening (refined oil blend, Uni-President). Freeze-dried mycelia of A. blazei, A. camphorata, H. erinaceus and P. linteus were obtained from the Biotechnology Centre, Grape King Inc., Chungli, Taiwan and were ground in a mill (RT-30HS, Rong Tsong Precision Technology Co., Taichung, Taiwan), and screened through a 0.5 mm sieve.