In the paper, a 20% erythrocyte suspension was incubated
with PBS (control) or preincubated with 5-HMF at the
indicated concentration at 37 °C for 20 min and then coincubated
with or without 100 mM AAPH at 37 °C for 2 h.
The hemolysis rate of erythrocytes by 5-HMF at 48.0 mM was
2.47 ± 0.006%, close to the control (1.95 ± 0.003%)