UV spectroscopy
The principles of UV spectroscopy were enumerated
earlier and will serve to quantify many of the subsequent
physical constants and solubilities above.
TLC, and particularly HPLC, has largely superseded
UV spectroscopy as a basic analytical tool in
stability assessment. However, certain UV techniques
are worthy of discussion:
• Solubility
• Molecular weight
' pKa
• Assay (potency)
• Mixtures:
- resolving compound products
- stability: hydrolysis, oxidation (coloured
products).