The particle size and charge of these vesicles/DNA complexes were characterized. Moreover, the physical stability of these cationic vesicles was evaluated by particle size and charge. For cationic niosomes, Span20-niosomes showed higher transfection efficiency and lower cytotoxicity than those of Tween61-niosomes. In case of cationic liposomes, the DOPE-liposomes showed the higher transfection efficiency than Chol-liposomes with similar cytotoxicity. Among the cationic lipids, high transfection efficiency was obtained from Ay in niosomes (ni-Sp20-Ay; Span20:Chol:Ay = 2.5:2.5:0.5 mM) and Dy in liposomes (li-PC-