3. Results and discussion
3.1. Batch enzymatic hydrolysis of cellulosic residue
3.1.1. Effects of substrate concentration
The effects of substrate concentration on enzymatic hydrolysis were investigated at a fixed ratio of cellulase from T. reesei ZU-02 to substrate (20 FPU g−1 substrate; 1.64 CBU g−1 substrate). As shown in Fig. 1, reducing sugar concentration and hydrolysis yield showed an opposite variation trend, i.e., with substrate concentration increasing, the reducing sugar concentration increased but the hydrolysis yield decreased. This may be due to the end-product feedback inhibition caused by high reducing sugar concentration (Wen et al., 2004). Taking reducing sugar concentration and hydrolysis yield into account, the optimal substrate concentration was 100 g l−1.
3. Results and discussion3.1. Batch enzymatic hydrolysis of cellulosic residue3.1.1. Effects of substrate concentrationThe effects of substrate concentration on enzymatic hydrolysis were investigated at a fixed ratio of cellulase from T. reesei ZU-02 to substrate (20 FPU g−1 substrate; 1.64 CBU g−1 substrate). As shown in Fig. 1, reducing sugar concentration and hydrolysis yield showed an opposite variation trend, i.e., with substrate concentration increasing, the reducing sugar concentration increased but the hydrolysis yield decreased. This may be due to the end-product feedback inhibition caused by high reducing sugar concentration (Wen et al., 2004). Taking reducing sugar concentration and hydrolysis yield into account, the optimal substrate concentration was 100 g l−1.
การแปล กรุณารอสักครู่..
