To evaluate the antimicrobial activity of the active films against
different strains in liquid media, different tubes containing 6 mL of
sterile saline solution with 10 strips (1 cm 5 cm) of active or
control films were uniformly inoculated with 1 mL of microbial
suspensions and incubated at room temperature for 3 days. After 0,
1 and 3 days, the tubes were cultured on a microorganism-specific
solid agar. Plate Count Agar was used for the bacterial counting and
Sabouraud Cloranfenicol Agarwas employed for moulds and yeasts.
The plates were incubated at 37 C for 24e48 h for bacteria or at
25 C for 72 h for moulds and yeast under aerobic or anaerobic
conditions depending on the microorganism, and then microbial
colonies were counted to evaluate the growth of the strains. The
microbiological data were transformed into the logarithm of the
number of colony forming units (CFU mL1). All determinations
were performed in triplicate.