A 100 L portion of these cultures was diluted to 50 mL liquid culture.
These inoculums were incubated for 5.5 h (for E. coli) and 6 h (for S. aureus) at 37 ◦C and 200 rpm rotation
A 1000 L portion of the diluted inoculation was added to a tube and diluted by taking 1 mL from a tube and adding another 1 mL sequentially as if all inoculums were diluted to 10−4 M.
100 L of inoculum was applied to the LB-agar medium and dispersed.