1.Bacterial colonies are suspended in sterile distilled water to a
turbidity of 0.5 McFarland (ca. 1.5 × 108 CFU/mL). Heat the
bacterial cell suspension to 100 °C for 15 min and then centrifuge at 11,000 ×g for 1 min. Transfer the supernatant of each
samples into a new labeled centrifuge tube and use as DNA
template or store at −20 °C. The supernatant can be used as
DNA template directly without further purification.