One percent tryptophan broth in a test tube was inoculated with bacteria colony. After incubation period of 37°c for 48hours, then one militre (1ml) of chloroform was added to the broth. The test tube was shaken gently, then 2.1 of Kovac’s reagent were added and this was also shaken gently and allowed to stand for twenty (20) minutes. The formation of red colouration at the top layer indicated positive and yellow colouration indicates negative